According to the regulations of the Ministry of Health, the HIV antibody positive report must be issued by an HIV antibody confirmation laboratory certified and qualified by the Ministry of Health to have legal effect. The most commonly used HIV antibody confirmation experimental method is Western blotting (Western Blot, WB). Evaluation of HIV antibody confirmation test results: The results of immunoblotting test are based on the different band types appearing on the specific HIV antigen positions of the nitrocellulose membrane to judge HIV antibodies as positive, negative and indeterminate.